Personal Information

 Assistant Professor

Department of  Laboratory Technology

Faculty of Applied Medical Sciences

Contact Information

Phone: 02-6402000

Email: wtounsi@kau.edu.sa

Wajnat Ali H. Tounsi

 Assistant Professor

Profile

Assistant professor at Faculty of applied medical sciences, department of medical laboratory sciences. I’m an enthusiast, attentive, and skilled young scientist. I completed my PhD from University of Plymouth, UK in 2021. My work evolved around blood group genotyping using different high throughput sequencing platforms including first, second, and third generation sequencing. I’m also a passionate teacher and I aspire to teach and convey the knowledge and experience I gained through my study. 
 

Education

  • 2006

    Bachelor degree from Laboratory MedicinceMedical Sciences, Um Al-Qura, Makkah, المملكة العربية السعودية

  • 2013

    Master degree from Medial Laboratory Science Health Science, Quinnipiac, Hamden, CT, امــريــكـا

  • 2021

    Doctorate degree from Biomedical SciencesSchool of Biomedical Sciences, University of Plymouth, Plymouth, بريطانيا

Employment

  • 2008-2015

    Teaching assisstant, King Abdulaziz University, جدة, المملكة العربية السعودية

  • 2015-2021

    Lecturer, King Abdulaziz University, جدة, المملكة العربية السعودية

  • 2022-حاليا

    Assisstant professor , King Abdulaziz University, Jeddaj, المملكة العربية السعودية

Research Interests

Research Interests

  • Rh blood group genes sequencing
  • Blood group genotyping
  • high throughput sequencing
  • next-generation sequencing (NGS)
  • third generation sequencing
  • MinION sequencing
  • Nanopore sequencing
  • Blood group genes zygosity determination
  • Establishment of haplotype specific reference blood group genes sequences
  • High throughput sequencing data analysis
  • MinION data analysis using command ubuntu

 

Publications

Wajnat A Tounsi, Vasileios P Lenis, Silja M Tammi, Susanna Sainio, Katri Haimila, Neil D Avent, Tracey E Madgett. Rh Blood Group D Antigen Genotyping Using a Portable Nanopore based Sequencing Device: Proof of Principle (2022). Clinical Chemistry: 1–6. Available at https://doi.org/10.1093/clinchem/hvac075

Silja M. Tammi*, Wajnat A. Tounsi*, Susanna Sainio, Michele Kiernan, Neil D.Avent, Tracey E. Madgett, Katri Haimila. Next-generation sequencing of 35 RHD variants in 16,253 serologically D negative pregnant women in Finnish population (2020). Blood Advances 4(20),4994- 5001. * Joint first authors. Available at DOI: 10.1182/bloodadvances.2020001569 

Wajnat A. Tounsi, Tracey E. Madgett, and Neil D. Avent. Complete RHD next generation sequencing: establishment of reference RHD alleles (2018). Blood Advances 2(20), 2713-2723. Available at https://doi.org/10.1182/bloodadvances.2018017871

Kelly A. Sillence, Amr J. Halawani,Wajnat A. Tounsi, Kirsty A. Clarke, Michele Kiernan, Tracey E. Madgett, and Neil D. Avent (2017). Rapid RHD zygosity determination using digital PCR. Clinical Chemistry 63(7), 1388-1397. Available at DOI: 10.1373/clinchem.2016.268698

 

Presentations

  • ISBT 36th international congress, virtual 2020. Poster presentation titled: sequencing of the RHD gene using MinION™.
  • University of Plymouth Annual postgraduate research event. Devon, United Kingdom. March 2019. Poster presentation titled: complete sequencing of the RH blood group genes using next generation sequencing.
  • Research and development Conference. Devon, United Kingdom. June 2018. Poster presentation titled: complete next generation sequencing for Rh genes: establishment of reference RHD and RHCE alleles.
  • ISBT 35th international congress. Toronto, Canada. June 2018. Poster presentation titled: complete next generation sequencing for Rh genes: establishment of reference RHD and RHCE alleles.
  • University of Plymouth annual postgraduate research event. Devon, United Kingdom. March 2018. Oral presentation titled: complete sequencing of the RH blood group genes using next generation sequencing.
  • ISBT 27th regional congress. Copenhagen, Denmark. June 2017. Poster presentation titled: complete sequencing of the RHD blood group gene using next generation sequencing: determination of RHD reference sequences.
  • University of Plymouth Annual postgraduate research event. Cornwall, United Kingdom. March 2017. Poster presentation titled: complete RHD next generation sequencing: establishment of reference RHD allele sequences.
  • University of Plymouth annual postgraduate research event. Cornwall, United Kingdom. March 2016. Poster presentation titled: investigation of intronic mutations in the RHD blood group gene.

Scientific interests

  • Rh blood group genes sequencing
  • Blood group genotyping
  • high throughput sequencing
  • next-generation sequencing (NGS)
  • third generation sequencing
  • MinION sequencing
  • Nanopore sequencing
  • Blood group genes zygosity determination
  • Establishment of haplotype specific reference blood group genes sequences
  • High throughput sequencing data analysis
  • MinION data analysis using command ubuntu

Courses

Areas of expertise

Blood group genotyping Blood group genotyping using NGS and TGS
Next generation sequencing Using next-generation sequencing for Blood group genes sequencing
Third generation sequencing (MinION) Exploring the possibilities of using third generation (TGS) sequencing (MinION) in blood group geneotyping
جينات فصائل الدم باستخدام التسلسل عالي الإنتاجية التنميط الجيني لفصيلة الدم باستخدام تسلسل عالي الكفاءة بما في ذلك تسلسل الجيل التالي وتسلسل الجيل الثالث.
إعداد مكتبة تسلسل الإنتاجية العالية إعداد مكتبة التسلسل لتسلسل الجيلين التالي والثالث